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KMID : 0438219760130020277
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Korea University Medical Journal
1976 Volume.13 No. 2 p.277 ~ p.285
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A Study on the Changes of Free Amino Acid Patten in Serum and Succus Entericus of Rat
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Abstract
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This study was carried out to observe the changes on metabolism of endogenous protein in animal which was maintained with protein free diet.
The animal, 72 adult male albino rats, 350 9^-390 g of body weight, were used for the experiments, and they were divided into two diet groups, the control and the protein free diet groups, and the latter group was again divided into 7 groups depend on feeding term: 2, 4, 7, 14, 24, 42 and 62-day-group.
The changes of the body weight were observed for 62 days and the values of total nitrogen and the free amino acid contents in the serum and the gut content were determined by the method of micro Kjeldahl and gas-liquid chromatography respectively.
The results obtained are summerized as follows.
1. The body weights were decreased gradually through the feeding periods of protein free diet, then decreased 42% to 46% of initial body weight during 62 days feeding of the diet.
2. The values of total nitrogen in the serum and the gut contents were similar to that of the control group during feeding of protein free diet for 24 days, then that of the serum was maintaintained to almost constant levels until 62-day, but that of the gut content was increased in 24-day to 62-day.
3. The changes of free amino acid contents:
a) The values of the total free amino acid in the serum were higher than the control group until 14 days feeding of the protein free diet, but there-after the values were decreased slowly, then 62-day-group have been lower value than the control group. Whereas, the values of total amino acid in the gut content were lower than that of the control group until 7 days feeding, after 42 days feeding, the values were shown a increased tendency.
b) The values of isoleusine and leucine in serum were similar to that of the control group for 42 days feeding of the protein free diet, but the values were lower than the control group in 62-daygroup. While, the both values in the gut content were lower than the control group for 14 days feeding, but there-after the values were increased to higher values than control group.
c) The value of lysine in the serum was slightly lower than the control group for 24 days feeding of the protein free diet, but the value was maintained to similar level with the control group in 42-day-group. While, the value of lysine in the gut content was shown similar tendency with the change in serum except after 42-days
d) The changes of methionine values in serum and gut content were contrary to each other throughout the period.
e) The values of phenylalaninc in scrum and gu content Nvere kept io similar value of the control group until 42 days feeding.
f? Threonine value in serium was decreased continuously for all feeding period, then the value of 62-day-group was bocome to half value of the control group´ While the value of the gut content v. as similar to value of the control group until 24 days feeding, there-after the value was increased.
g) The values of tryptophan in serum and gut content were trace level during 42 to 62 days feeding of protein free diet.
h) The values of alanine, glutamic acid, glycine, serine and proline in serum were significantly higher than that of control group during 14 to 24 days feeding of protein free diet, but the values j of 42-day-group were decreased to levels of the control group.
In view of above results, it would be empasized that the ability of metabolic homeostasis was relatively well behaved for 7^14 days, there-after, the ability was decreased by feeding of protein j free diet, and the ability in gut homeostasis was contrary to metabolic homeostasis.
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